Blues will play one of their home fixtures in Group H at Loughborough University Stadium.
Leeds United, Derby County and Wolverhampton Wanderers are their opponents, with the teams meeting home and away.
Blues begin their fixtures against Leeds at Tadcaster Albion FC on 4 October (7.30pm).
Then it's another away trip, to face Wolves, at AFC Telford United on 9 November (1pm).
Match Round 3 sees an away game against Derby, which is also at Loughborough University Stadium (2 December, 7pm).
Blues face Leeds at home on 17 January, 2020 (2pm) - the venue has yet to be confirmed - and Wolves at home at the Loughborough University Stadium on 6 February, 2020 (2pm).
Blues last game is against Derby on 21 February (2pm), with the home venue also yet to be confirmed.
The top two teams in each group - there are eight groups in total - qualify for the knockout stage of the competition.
* Blues Under-23s next PDL match is against Sheffield United Under-23s at the St. Andrew's Trillion Trophy Stadium on Monday (1.30pm).